Reproductive Toxicity Screen of 1,3,5-Trinitrobenzene Administered in the Diet of Sprague-Dawley Rats.
Abstract
Several Army installations targeted for restoration have measurable quantities of 1 ,3,5-trinitrobenzene (TNB) in the soil and ground water. As part of the process to develop environmental and health effects criteria for restoration, a modified Screening Information Data Set (SIDS) reproductive study was performed. Male and female Sprague-Dawley rats received diet containing approximately 300, 150, or 30 mg Th%B/kg diet. Mating occurred following 14 days of treatment. All dams, one-half the males, and representative pups were maintained for a total of 90 days of treatment. No mortality occurred during the study; however, a decrease in mean body weights was noted in both sexes of high-dose rats. A dose-related effect was noted in measurements of sperm function/activity. Sperm depletion and degeneration of the seminiferous tubules were noted histopathologically. Methemoglobinemia and splenic hemosiderosis were common findings in the high- and mid-dose levels of both sexes at necropsy. No adverse effects were noted in mating or fertility indices. No significant treatment-related differences were found in length of gestation, sex ratio, gestation index, or mean number of pups per litter.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1994
- Accession Number
- ADA298912
Entities
People
- C. D. Flemming
- C. R. Miller
- D. J. Caldwell
- E. R. Kinkead
- R. E. Wolfe