Metabolism of 2,2-Dichloro-1,1,1 Trifluoroethane (HCFC-123) by Human Hepatic Microsomes.
Abstract
As part of its safety evaluation, the in vitro metabolism of 2,2-dichloro-1,1,l-trifluoroethane (HCFC-123), a replacement candidate for Halon 1211, by human hepatic microsomes, was assessed. Microsomal incubations containing HCFC-123 ranging from 6 to 75% (v/v) in the headspace produced increasing amounts of trifluoroacetic acid (IFA); the kinetics suggested substrate- saturation although substrate inhibition was apparent above a concentration of 36%. The rate of ThA formation with respect to pH, time, and protein concentration permitted linear rates of formation to be determined. Rates of ThA formation from incubations conducted at physiological pH, and containing concentrations of HCFC-123 in solution representing estimated concentrations of the chemical in human liver at steady-state, were 67% of those obtained under optimal conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1994
- Accession Number
- ADA299025
Entities
People
- A. Vinegar
- C. S. Godlin
- J. M. Drerup
- M. M. Ketcha