Alternative Splicing in Normal Development and in Breast Cancer.
Abstract
Alterations in the splicing patterns of key regulatory genes are likely to play an important role in oncogenesis. The ASF/SF2 protein is one of a family of SR splicing factors that have been shown to regulate splice site choice in vitro. We have mapped the ASF/SF2 gene to 17q21.3-q22 in humans, and close to the Ovum mutant locus on chromosome 11 in mice. Our current objective is to examine the role of the ASF/SF2 gene in development and oncogenesis by observing the effects of disrupting the gene in mice. We have generated seven embryonic stem (ES) cell lines that are heterozygous for a deletion of the ASF/SF2 gene. Chimeric mice have been generated for four of these cell lines, but we have not yet achieved germ line transmission of the ASF/SF2 mutation. It is currently unclear whether this result is due to difficulties in the handling and/or injection of the ES cells, or is due to haploinsufficiency of the ASF/SF2 gene.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 28, 1995
- Accession Number
- ADA299265
Entities
People
- John R. Bermingham
Organizations
- University of California, San Diego