Mapping of a Breast Carcinoma Tumor Suppressor Gene to Chromosome 11p15.5.
Abstract
The identification of human tumor suppressor genes has led to new insights into the mechanisms of human cancer development. Most of the known tumor suppressor genes were isolated by determining their chromosomal location using molecular markers or cytogenetics. By this manner, several reports have mapped a tumor suppressor gene for human breast cancer to a region of chromosome l1p15.5. Our preliminary physical map of this area suggests that this region contains less than 1000 kilobases (kb). During the last year, we have gathered cosmids, P1s and yeast artificial chromosomes (YACs) from this area for the isolation of new polymorphic markers. We have also obtained matched DNA samples from over 25 normal breast and breast tumors during the past year. During the next funding period, we plan to initiate the identification of new di- tri- and tetra-nucleotide repeats from our genomic inserts in the region. We will also start loss of heterozygosity studies to further narrow the tumor suppressor region. The availability of a novel tumor suppressor gene inactivated in 25% of breast cancers would lead to new methods for treatment and early detection and may provide important clues about the process of normal mammalian tissue development
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 26, 1995
- Accession Number
- ADA299344
Entities
People
- Bernard E Weissman
- Perry Gasque-carter
Organizations
- University of North Carolina at Chapel Hill