Generation of Novel Protein Receptors.
Abstract
Families of protein receptors will be created from a small, stable template. The experimental approach involves synthesizing the gene for the template protein and cloning it into a bacteriophage vector to allow phage display of the protein. In this way, replication and recognition can be coupled. Repeated rounds of mutagenesis and affinity chromatography will be employed to select the desired binding properties out of the diverse population created from the original protein scaffold.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 30, 1994
- Accession Number
- ADA299460
Entities
People
- Donald Hilvert
Organizations
- Scripps Research