Molecular Diagnosis for Breast Malignancy.
Abstract
This grant investigates a major surface protease complex of malignantly transformed cells (malignancy antigens), containing maligrin and seprase, as indicators of the metastatic potential of breast cancer cells. The aims are to evaluate conceivable invasion-related molecules as prognostic markers for node-negative breast cancer. During the first year of this grant, we have identified the p29 maligrin-associated protein and its corresponding cDNA, selected monoclonal antibodies useful in staining of paraffin sections, and established monoclonal antibody sandwich ELISA technique using breast cancer cells in culture (6 cell lines) as proposed in the Statement of Work. MDA-MB-231 cDNA library was initially screened with anti-p29 antibodies. Positive clones were sequenced and identified as a 58-kDa human tumor derived collagenase-stimulating factor, now called EMMPRfN. The p29 antigen was originated from bovine semm as identified by the sandwich ELISA, and it bound to cancer cell surface invadopodia. Future isolation and expression of EMMPRfN will be sought to test the recombinant molecule in stimulating production of
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 28, 1995
- Accession Number
- ADA299495
Entities
People
- Wen-tien Chen
Organizations
- Georgetown University