Function of the Stroma-Derived Metalloproteinase, Stromelysin-3, in Invasive Breast Carcinomas.

Abstract

Stromelysin-3, a new member of the matrix metalloproteinase gene family, is specifically expressed in stromal cells surrounding invasive breast carcinoma cells, but its function has remained largely undefined. We now report that human stromelysin-3 is processed from an inactive zymogen to an active endoprotease following intracellular activation by the proprotem convertase, furin. Active stromelysin-3 is then secreted extracellularly where it is able to cleave and inactivate members of the serine proteinase inhibitor family. Given the fact that breast cancer cells have recently been shown to secrete powerful proteinase (e.g., leukocyte elastase, plasmin) whose activities are normally regulated by serine proteinase inhibitors, our data suggest that stromelysin-3 may regulate breast cancer cell function by controlling the local proteinase-antiproteinase balance.

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Document Details

Document Type
Technical Report
Publication Date
Jul 27, 1995
Accession Number
ADA299781

Entities

People

  • Stephen J. Weiss

Organizations

  • University of Michigan

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Anti-Bacterial Agents
  • Biomedical And Dental Materials
  • Blood
  • Breast Cancer
  • Cell Line
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Culture Media
  • Epithelial Cells
  • Gene Expression
  • Medical Personnel
  • Neoplasms
  • Proteins
  • Sequence Analysis

Fields of Study

  • Computer science

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biochemistry