Electrostatic Control of Acetylcholinesterase Reactivity.
Abstract
These studies examined chiral reactivity, the magnitude and importance of steric interactions, and the importance of the electrostatic charge distribution in governing reactivity of acetylcholinesterase (AchE). To do this, we employed a comprehensive series of resolved enantiomeric methylphosphonates, a comprehensive series of alkylphosphonates, and fluorescent methylphosphonates.As a complementary index to detailed stopped-flow analysis of inhibition kinetics, we monitored kinetics of aging and oxime reactivation. Together with equilibrium binding studies, employing the reversible fluorescent ligand decidium diiodide, these provided an independent index of ligand orientation achieved by the organophosphonates within the enzyme active center.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1995
- Accession Number
- ADA299909
Entities
People
- Harvey A. Berman
Organizations
- University at Buffalo