Progress Report for Contract N00014-94-C-0015 (AT and T Bell Lab).

Abstract

Our main goal for the next year is to perform optical recordings with single-cell resolution of a population of neurons in the adult cortical slice These experiments did not worked initially because of the failure of calcium indicators to label populations of adult neurons. Nevertheless, preliminary experiments by us and a number of groups suggest that extracellular injections of dextrans coupled to calcium indicators can be used to labeled populations of neurons in a variety of Systems. Other possible approaches are the use of calcium indicators with less acetoxymethyl esters or the use of a new generation of voltage-sensitive dyes that can be transported retrogradely or that have much larger signals that previous ones. These different strategies appear to us very promising solutions to the problem.

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Document Details

Document Type
Technical Report
Publication Date
Feb 22, 1995
Accession Number
ADA299925

Entities

People

  • David W. Tank
  • Rafael Yuste

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amplifiers
  • Biological Sciences
  • Biology
  • Brain
  • Computations
  • Computer Simulations
  • Current Amplifiers
  • Demographic Cohorts
  • Electrophysiology
  • High Resolution
  • Indicators
  • Neurosciences
  • Simulations

Readers

  • Molecular and Cellular Biochemistry
  • Neuroscience
  • Regression Analysis.