Evaluation of Dried Storage of Platelets for Transfusion: Physiologic Integrity and Hemostatic Functionality.
Abstract
Currently, therapeutic platelet concentrates can be stored for only 5 days. We have developed a procedure that permits long term storage of fixed and lyophilized platelets that retain hemostatic properties after rehydration. These rehydrated lyophilized platelets (RL platelets) restore hemostasis in thrombocytopenic rats and become incorporated in the hemostatic plug of bleeding time wounds of normal dogs as well as von Willebrand disease dogs with partially replenished plasma von Willebrand factor. Ultrastructurally these platelets are well preserved and are comparable to control normal washed platelets. Flow cytometry analysis shows that RL platelets react with antibodies to the major surface receptors glycoprotein (GPIlb and GPIlb/IIIa. These receptors are involved in platelet agglutination, aggregation, and adhesion. In vitro functional tests document the ability of RL platelets to adhere to denuded subendothelium and to spread to C on a foreign surface. Circulating RL platelets participated in carotid arterial thrombus formation induced in normal Carotid subjects. The participation of RL platelets in these vital hemostatic properties suggests that with further development they could become a stable platelet
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1995
- Accession Number
- ADA299966
Entities
People
- Arthur P. Bode
- Majorie S. Read
- Robert L. Reddick
Organizations
- East Carolina University