Identification of Brucella Melitensis Antigens Lymphocytes and Oral Immunization Potential of the Antigens.
Abstract
We have produced substantial results related to the objectives of our Department of Defense funding: (1) Identified five Brucella proteins and sequenced their respective genes (L71L12, LlO, uvrA, SSB, and a transporter containing an ATh binding domain) - employing a rapid and dIrect technique to isolate Brucella genes from a genomic library using lymphocytes as probes and obtained two additional genes (GroEL and GroES) whose proteins also stimulate lymphocyte proliferation. (2) Our recently published data indicates we have successfully identified, isolated, cloned, and sequenced genes and expressed proteins that induce proliferation of lymphocytes from many Brucella immunized animals suggesting immunenodominant proteins. (3) We now have unprecedented data establishing CD8+ T cells as essential to protection against B. abortus infection using MMC class II gene knockout mice. These knockout mice are profoundly deficient in CD4+ T cells but rapidly clear the infection, revealing the pivotal role of CD8+ T cells in protection. Our murine experiments provide the necessary foundation to explore the mechanisms of Brucella pathogenesis and in vivo protection attributable to Brucella proteins that induce T cell responses in vitro. We are positioned to apply this practical information to vaccine studies for protection against B. melitensis challenge and provide critical data necessary for a human vaccine.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 31, 1995
- Accession Number
- ADA300038
Entities
People
- Gary Splitter
Organizations
- University of Wisconsin–Madison