Molecular Determinants of Antiestrogen and Drug Sensitivity in Breast Carcinoma Cells.
Abstract
NCF7 breast carcinoma cells were rendered susceptible to infection with recombinant ecotropic retroviruses, through transfection with the gene for murine ecotropic receptor and by testing individual transfectants for infectability. A genetic suppressor element (GSE), derived from the BCL2 gene which inhibits active cell death (apoptosis), was introduced into NCF7 cells. The BCL2-derived GSE sensitized MCF7 cells to different chemotherapeutic drugs, indicating that BCLt2 is an important determinant of chemoresistance of these cells. A retroviral library of normalized cDNA fragments, derived from Heta cell line, was introduced into NCF7 cells, and selection of GSEs that render MCF7 cells resistant to doxorubicin was initiated. A normalized cDNA library is also being prepared from NCF7 cells, for use as a source of GSEs conferring resistance to tamoxifen.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 14, 1995
- Accession Number
- ADA300046
Entities
People
- Igor B Roninson
Organizations
- University of Illinois at Chicago