Genomic Instability at Premalignant and Early Stages of Breast Cancer Development
Abstract
We have developed techniques that allow the analysis of multiple chromosomal loci from single paraffin sections from breast cancer lesions. This approach is being used to allelotype small preinvasive breast cancer lesions, correlating with other parameters such as specific gene mutations and expression and with interphase cytogenetics on the same lesions. We identified the chromosome arms most frequently affected by allelic losses and imbalances at preinvasive stages of breast carcinogenesis and those allelic losses involved in more advanced stages of progression. We are continuing with these studies and further refining the mapping of specific subchromosome areas involved in early breast tumorigenesis. In the course of these studies we observed that microsatellite instability (replication error phenotype, RER+) is characteristic of a subset of breast tumors (invasive lobular breast carcinomas), indicating the possible involvement of defective DNA mismatch repair genes in these tumors. Studies are ongoing to address this point and to evaluate the use of RER phenotype determination as a prognostic-diagnostic tool.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1995
- Accession Number
- ADA300054
Entities
People
- C M Aldaz
Organizations
- University of Texas at Austin