New Therapeutic Approaches and Prognostic Assays for Breast Cancer: Radiolabeled Ligans and Antibodies and Quantitative PCR.

Abstract

Our research proposal is on developing highly sensitive, rapid, cost-effective molecular assays for quantitating the transcripts of prognostic factors in breast cancer. In the first granting period, our goals were to establish the conditions for quantitating the transcripts of wild type-, and variant types of estrogen receptor (ER) using breast cancer cell lines in culture. We have designed PCR primers which can amplify various portions of ER mRNA. We tested the primers first on a plasmid cDNA (plC-ER-F) which contains full length coding sequences of ER to establish the optimum PCR conditions. We have then used them to amplify various exons of ER cDNA prepared by reverse transcribing the RNA from MCF-7 cells. We are currently in the process of quantitaing the ER transcripts in MCF-7 cells by competition assay.

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Document Details

Document Type
Technical Report
Publication Date
Aug 28, 1995
Accession Number
ADA300118

Entities

People

  • Indra Poola
  • Robert E. Taylor

Organizations

  • Howard University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Diseases And Disorders
  • Estrogens
  • Gene Expression
  • Hormones
  • Laboratory Animals
  • Mammary Glands
  • Molecules
  • Neoplasms
  • Polymerase Chain Reaction
  • Proteins
  • Sequences
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics