Characterization of CTL Recognized Epitodes on Human Breast Tumors.

Abstract

The overall objective is to develop novel therapeutic approaches to breast cancer by understanding the molecular and cellular basis of HER-2 recognition on breast tumor cells by cytotoxic T lymphocytes (CTL). To optimize CTL recognition of antigenic epitopes on HER-2 we defined the role of residues in the epitopes in enhancing HLA-binding and target lysis. We found that in the HLA-A2 system positions PI - P3 are more amenable to changes which stabilize binding and do not affect CTL recognition. We are now characterizing tumor-bound peptides through immuno-affinity elution, HPLC, and defining peptides that can reconstitute common Ag which may be clinically relevant. To address the nature and frequency of ex vivo CTL responses to breast tumors and HER-2, we are developing CD8+ CTL lines and clones from lymphocytes infiltrating primary or metastatic breast tumors and defining their specificity of Ag recognition in functional assays.

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Document Details

Document Type
Technical Report
Publication Date
Sep 18, 1995
Accession Number
ADA300474

Entities

People

  • Constantin G. Ioannides

Organizations

  • University of Texas at Austin

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Lymphatic System
  • Lymphocytes
  • Medical Personnel
  • Oncology
  • Peptide Growth Factors
  • Proteins

Fields of Study

  • Biology
  • Medicine

Readers

  • Mathematical Modeling and Probability Theory.
  • Molecular and Cellular Biochemistry
  • Oncology