Novel Approaches to the Characterization of Specific Protein-Protein Interactions Important in Gene Expression.

Abstract

Through the application of a range of biochemical, immunochemical and genetic techniques, we have accomplished the following goals: First, we characterized a previously undescribed protein, WrbA and showed that it functions as an accessory protein in the binding of TrrR to its operator target; second, we demonstrated that WrbA is a stationary-phase protein, and that the regulation of promoters controlled by TrpR becomes more stringent in stationary phase cells; third, we developed a method for the purification of the TyrR protein, a dual-specificity transcription factor that can both activate and inhibit the action of RNA polymerase. The domain structure of TyrR was established by chemical procedures.

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Document Details

Document Type
Technical Report
Publication Date
Oct 14, 1994
Accession Number
ADA300480

Entities

People

  • Ronald L. Somerville

Organizations

  • Purdue Research Foundation

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Aromatic Amino Acids
  • Chemical Reactions
  • Chemistry
  • Escherichia
  • Escherichia Coli
  • Gene Expression
  • Genetic Code
  • Genetic Techniques
  • Genetics
  • Hydrolysis
  • Mrna
  • Prokaryotes
  • Protein-Protein Interactions
  • Proteins
  • Regulations

Fields of Study

  • Biology

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology