Progress Report for Contract Number N00014-93-C-0019. Quarterly Report Nomber 8. (Hawaii Biotechnology Group, Inc.).

Abstract

(1) The production of anti-ferret IgA antibodies. Status: Immunization of rabbits and collection of sera completed. Mouse ascites successfully cross-adsorbed; material sent to NMRI researchers in 12/94. (2) The purification of lipopolysaccharide from Sbigella and Campylobacter species. Status: Shigella sonnei and S. flexneri LPS delivered to NMRI researchers. Purification of C. jejuni LPS reinstated. Have collected 173mg of required a 200mg LPS. Production of remainder in progress. (3) The development of an enzyme immunoassay for the detection of enteroaggregative Escherichia coli heat-stable toxin. Status: Initial recombinants provided by Navy unsatisfactory. Work suspended in 10/93 due to contract funding reduction. Suitable recombinants still not available. (4) The production of monoclonal antibodies against strain-specific antigenic epitopes on Campylobacter coli flagella. Status: Completed 3 fusions. No strain specific MAbs identified.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 1994
Accession Number
ADA300539

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Bacteria
  • Biological Toxins
  • Biomedical And Dental Materials
  • Cells
  • Cellular Structures
  • Diseases And Disorders
  • Escherichia Coli
  • Gram-Negative Bacteria
  • Immune Serums
  • Immunoglobulins
  • Lipopolysaccharides
  • Microbiology
  • Polymeric Films
  • Polysaccharides
  • Proteins

Fields of Study

  • Biology

Readers

  • Immunology
  • Microbial Pathology
  • Technical Research and Report Writing.

Technology Areas

  • Biotechnology