Novel Approaches to the Characterization of Specific Protein-Protein Interactions Important in Gene Expression.

Abstract

Through the application of a range of biochemical, immunochemical and genetic techniques, we have accomplished the following goals: First, we characterized a previously undescribed protein, WrbA and showed that it functions as an accessory protein in the binding of TrpR to its operator target; second, we demonstrated that WrbA is a stationary-phase protein, and that the regulation of promoters controlled by TrpR becomes more stringent in stationary phase cells; third, we developed a method for the purification of the TyrR protein, a dual-specificity transcription factor that can both activate and inhibit the action of RNA polymerase. The domain structure of TyrR was established by chemical procedures.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 14, 1995
Accession Number
ADA300572

Entities

People

  • Ronald L. Somerville

Organizations

  • Purdue Research Foundation

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Aromatic Amino Acids
  • Chemical Reactions
  • Chemistry
  • Escherichia
  • Escherichia Coli
  • Gene Expression
  • Genetic Code
  • Genetic Phenomena
  • Genetic Techniques
  • Genetics
  • Mrna
  • Prokaryotes
  • Protein-Protein Interactions
  • Proteins
  • Regulations
  • Stationary

Fields of Study

  • Biology

Readers

  • Molecular Genetics

Technology Areas

  • Biotechnology