Programmed Cell Death in Breast Cancer.

Abstract

E HAVE INITIATED STUDIES OF PROGRAMMED CELL DEATH, OR POPTOSIS, IN HUMAN BREAST CANCER CELLS. Using the MCF-V reast cancer cell line, we have found that cell growth is stimulated by supplementing the culture medium with l%%-estradio1, and inhibited by charcoal stripping serum o remove steroid hormones. We have found that MCF-% cells are resistant to induction of apoptosis by the calcium pump inhibitorr thapsigargin, ostensibly due to expression of Bcl-2. We have found that estrogen ithdrawal decreases the level of Bcl-2 expression, but does not increase the susceptibility of cells to apoptosis due to constitutive Bcl-2 expression.

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Document Details

Document Type
Technical Report
Publication Date
Aug 28, 1995
Accession Number
ADA300581

Entities

People

  • Clark W. Distelhorst

Organizations

  • Case Western Reserve University

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Benefits
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Cultured Cells
  • Fringe Benefits
  • Health Services
  • Indirect Costs
  • Inhibitors
  • Laboratory Animals
  • Law
  • Materials
  • Patient Care
  • Programmed Cell Death
  • Tumor Cell Line

Fields of Study

  • Biology
  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.