Tumor Suppressor Genes in Early Breast Cancer and Its Progression.

Abstract

Ductal carcinoma in situ (DCI S) of the breast is a pre-invasive form of breast cancer, which in some cases is a precursor to invasive ductal cancer. Many tumor suppressor loci are inactivated in invasive breast cancer, however little is known concerning the genetic events preceding invasion. Using polymerase chain reaction of microsatellite markers we have allelotyped 61 tumor/normal pairs from patients with DCIS. Tumor DNA template was obtained from microdissected archival samples. Significant loss of heterozygosity (LOll) was found for chromosomal loci on 8p (18.7%), 13q (18%), 16q (28.6%), 17p (37.5%) and 17q (15.9%). The region of allelic loss on 8p has been examined with an expanded panel of markers on 64 tumors, and the smallest region of deletion narrowed down to 1.3 cM. A fine structure map of 8p is being created using genotypic data from CEPH families. Assay of twelve sets of synchronous in situ and invasive breast cancer revealed that clonal expansion takes place in the progression of DCIS and that loci on lip may be involved on the invasive phenotype.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1995
Accession Number
ADA301623

Entities

People

  • Diane M. Radford

Organizations

  • University of Washington

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Carcinoma
  • Chromosomes
  • Colon Cancer
  • Databases
  • Genes
  • Genetics
  • Genome
  • Health Services
  • Medical Personnel
  • Neoplasms
  • Oncology
  • Polymerase Chain Reaction
  • Statistical Analysis

Readers

  • Molecular and genetic basis of cancer.
  • Oncology and Biomarker-Based Cancer Detection.

Technology Areas

  • Biotechnology