Role of Mammary Adenocarcinoma Cell Transterrin Response In Breast Cancer Metastasis
Abstract
We have found that breast cancer cell expression of the transferrin receptor (TfR) and cellular ability to proliferate in response to transferrin (Tf) are properties which correlate with the ability of mammary cancer cells to metastasize. The current grant focuses attention on different mechanisms for elevating or depressing breast cancer cell TfR expression and to assess any changes in metastatic capability caused by these alterations. The current objectives are: (1) to raise tumor cell TfR levels by transfection with a sense TfR plasmid construct; (2) to decrease breast cancer cell TfR levels by transfection with an antisense TfR plasmid construct; (3) to select high or low TfR expressors by the use of various growth selection or FACS techniques. We have been able to increase breast cancer cell metastatic ability by the use of a Tf growth selection process. Transfection of tumor cells with a sense TfR construct has resulted in cells with markedly higher TfR expression. However, increased TfR expression does not necessarily cause an increased proliferative response to Tf. Transfection with a TfR antisense construct have not yet resulted in a depression of TfR levels. Studies which define cellular properties that link increased TfR expression to increased response to Tf are indicated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 21, 1995
- Accession Number
- ADA302310
Entities
People
- Philip G. Cavanaugh
Organizations
- University of Texas at Austin