PH Regulation by Breast Cancer Cells In Vitro and In Vivo.
Abstract
PH is a fundamental parameter of tumor progression. We are investigating the mechanisms regulating both the intracellular and extracellular pH of breast cancer tumors, both in vivo and in vitro. For extracellular pH studies, we are using 31P-NMR spectroscopy of human breast cancer tumors grown in nude mice. In these studies, extracellular pH is measured using an exogenous compound, 3-aminopropylphosphonate (3-APP), and intracellular pH is measured using endogenous inorganic phosphate. Over the past year, we have been refining these techniques, which are now in use by at least three other labs in the world. For measurement of intracellular pH, we are using fluorescence spectroscopy and molecular biological techniques to characterize the activity of plasma membrane associated Vacuolar type H+-ATPase (pV-ATPase). This pump is highly active in approximately half of the tumor cell lines investigated, and correlates highly with both invasive potential and drug resistance. We hypothesize that these are related and can be explained by a rapid rate of endomembrane recycling.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 29, 1995
- Accession Number
- ADA302477
Entities
People
- Robert J Gillies
Organizations
- University of Arizona