Cloning Human Chromosome 17 Genes:
Abstract
Our research interest is focused on the development of new strategies to identify genes from chromosome 17. The isolation of genes transcribed from chromosome 17 will provide candidates for the proposed sporadic breast and ovarian cancer genes. We have recently reported a method for the isolation of chromosome specific cDNAs using high density arrayed cDNA and chromosome specific cosmid libraries. The ability to isolate genes in a chromosome specific manner provides simultaneous identification of the expressed sequence and a chromosomal location. This new technology identifies expressed sequences by reciprocal probing of arrayed cDNA libraries and a chromosome specific cosmid library. We have used these resources to identify 1794 clones from the Los Mamos chromosome 17 cosmid library using probes generated from a placental cDNA library. So far, we have isolated and characterized 42 cDNAs to chromosome 17. Several of these cDNAs mapped to the region 17p13, while 14 other cDNAs mapped to the region of 17q12-22. Recent studies have demonstrated that in some sporadic breast cancer there are loss of heterozygosity (LOH) in these regions of human chromosome 17.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1995
- Accession Number
- ADA302601
Entities
People
- Cheng-chi Lee
Organizations
- Baylor College of Medicine