Canine Vocal Fold Fibroblasts in Culture: Expression of alpha-Smooth Muscle Actin and Modulation of Elastin Synthesis.

Abstract

Fibroblasts from canine vocal fold tissue have been isolated and maintained in culture. These cells express vimentin and a-smooth muscle actin, the characteristic marker of myofibroblast differentiation. Elastin production by vocal fold fibroblasts was compared to that of skin fibroblasts. The effects of transforming growth factor-Beta and hydrocortisone on elastin production were examined. Vocal fold fibroblasts in culture have a high elastogenic capacity compared to skin fibroblasts, and their elastin production can be enhanced by hydrocortisone (1.3uM) and TCF-Beta (10ng/ml). We conclude that the cells within the vocal fold are myofibroblastic in nature, and have high elastogenic potential in vitro. We suggest that connective tissue remodelling may be a continuous process in the vocal fold.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1991
Accession Number
ADA302739

Entities

People

  • Caroline Broadley
  • Debra A. Gonzalez
  • Jeffrey M. Davidson
  • Rhada Nair

Organizations

  • Vanderbilt University

Tags

DTIC Thesaurus Topics

  • Albumins
  • Anti-Bacterial Agents
  • Antibodies
  • Cells
  • Collagen
  • Connective Tissue
  • Epithelial Cells
  • Fibroblasts
  • Free Electrons
  • Growth Factors
  • Mucous Membrane
  • Muscle Cells
  • Muscles
  • Peptide Growth Factors
  • Proteins
  • Smooth Muscle
  • Tissues

Fields of Study

  • Biology
  • Medicine

Readers

  • Immunology and Pathology
  • Military Logistics and Supply Chain Management