Cellular and Molecular Mechanisms of High Pressure Inotropy in Cardiac Muscle.
Abstract
The objective of the project was to exploit isolated cardiac myocytes as a model system for investigating the effects of hydrostatic pressure on calcium regulated phenomena. This involved 5 specific aims: (1) to develop instrumentation to perform needed measurements of intracellular calcium transients jrf at high hydrostatic pressure and with high time-resolution; and to determine (2) if pressure acts to change resting and/or dynamic cytosolic-calciunrin-the mammallan cardiac myocyte, (3) the role of the sarcoplasmic reticulum in pressure induced inotropy, (4) the role of sodium-potassium pump inhibition in pressure induced inotropy, (5) the role of the sodium-calcium exchanger in pressure induced inotropy. Objective 1 was met with the development of the devices and techniques developed over the course of this project. Objective 2 was met with the initial design of the pressure chamber. Further refinements were required to meet the remaining objectives, which are currently being investigated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 04, 1995
- Accession Number
- ADA302891
Entities
People
- Perry M. Hogan
- Stephen R. Besch
Organizations
- University at Buffalo