Regulation of the Gl-S-Phase Transition in Non-Transformed and Transformed Human Breast Epithelial Cells.
Abstract
The goal of this work is to study the regulation of the thymidine kinase (TK) gene in non-tumorigenic and tumorigenic human breast epithelial cells. Previous studies in our laboratory have shown that this gene is regulated during the cell cycle by both transcriptional and post-transcriptional mechanisms. The approach we are using in these studies is to examine the expression of hybrid genes in stably transfected breast cancer cells. Two hybrid genes have been constructed thus far: (1) TK-Luc, which contains the human TK promoter linked to a luciferase reporter gene, will be used to study transcriptional regulation; (2) CMV-TK*, which contains an epitope-tagged human TK cDNA expressed from a constitutive CMV promoter, will be used to study post-transcriptional regulation. These constructs have been transfected into non-tumorigenic (184B5) and tumorigenic (MCF-7) human breast epithelial cells. We are currently in the process of isolating and characterizing stably transfected cell lines. These transfectants will then be analyzed for regulation of the transfected gene(s). The results of these studies will identify the mechanism(s) that regulate cell cycle specific gene expression in normal breast epithelial cells, and establish whether these mechanisms are disrupted during the process
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1995
- Accession Number
- ADA303143
Entities
People
- Samuel E. Conrad
Organizations
- Michigan State University