Regulation of Estrogen Receptor Transcription in Breast Carcinoma.
Abstract
An important transcriptional regulatory element of the estrogen receptor (ER) gene has been identified that binds a protein expressed in ER-positive breast carcinoma. Using a transient expression assay, a seventy-five base pair region of the 5' untranslated leader of the ER gene was identified which augments expression from the ER promoter.This region contains two binding sites for a protein, estrogen receptor factor-1 (ERF-1), which is expressed in ER-positive breast carcinomas. A concentrated ERF-1 binding site probe identified a 30,000 dalton protein. Low level ERF-1 expression was detected in normal human mammary epithelial cells. Abundant ERF-l expression was also found in endometrial carcinoma cell lines that express the ER-positive phenotype. These results indicate that ERF- 1 expression represents a common mechanism of ER regulation in hormonally responsive carcinomas.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1995
- Accession Number
- ADA303190
Entities
People
- Ronald J. Weigel
Organizations
- Stanford University