Suppression of Vascular Growth in Breast Cancer.
Abstract
Growth and metastasis of breast cancer is directly dependent on neovascularization. By Understanding the mechanisms that control the neovascular response, it may be possible to design therapeutic strategies to selectively prevent or halt pathological growth of vessels and consequently restrain the progression of cancer cells. Despite its general biological significance and pathological relevance, relatively little is known about inhibitors of blood vessel formation. Thrombospondin-l (TSPl) has recently been identified as a negative regulator of angiogenesis. Its relevance for the suppression of vascular growth in tumors has yet to be investigated. The present proposal was designed to address the role of TSPl in the neovascularization of mammary tumors. Initially, kinetics of vascular development will be examined in mammary tumors of TSP 1 - deficient mice and in tumors of control animals. A second set of experiments will focus on the effect of TSPl in normal and tumor-derived endothelial cells at the cellular and at the molecular level. Specifically, we will investigate the proliferation, invasion, and chemotaxis of normal and tumor-derived endothelial cells in a model of angiogenesis in vitro. A final facet of this proposal is directed to investigate the modulation of TSP 1 receptors as both populations of cells organize into cords and tubes in vitro and to identify the receptor(s) responsible for the generation of signals ultimately responsible for the regulation of endothelial cell behavior in breast cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1995
- Accession Number
- ADA303492
Entities
People
- Luisa Iruela-arispe
Organizations
- Beth Israel Deaconess Medical Center