The Role of P53 in Human Breast Cancer.

Abstract

The technical objectives of this project are to transfect mutant p53s into normal human mammary epithelial cells obtained from different donors and to isolate clones. The clones will be characterized for extension of lifespan and immortalization in cell culture. It will also be determined if expression of any of the mutant p53s provide a growth advantage to breast epithelial cells prior to immortalization. An additional objective is to determine in breast epithelial cells immortalized and expressing mutant p53 if expression of the mutant p53 is necessary for the maintenance of growth. Finally, downstream genomic targets of pS3 that may be important in the development and progression of breast cancer will be determined.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1995
Accession Number
ADA303793

Entities

People

  • Jerry W. Shay

Organizations

  • University of Texas at Dallas

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Count
  • Cell Line
  • Cells
  • Chemistry
  • Chromosomes
  • Culture Techniques
  • Epithelial Cells
  • Genes
  • Genetics
  • Growth Factors
  • Materials
  • Neoplasms
  • Papillomavirus Infections
  • Polymerase Chain Reaction
  • Proteins
  • Viruses

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Immunology and Pathology
  • Neurological Diseases/Conditions/Disorders