Multiple Gene Blotting to Screen for Cytokine RNA Expression: Application to Endothelioma Cells.

Abstract

BACKGROUND: Because normal and tumor cells can secrete different cytokines, it has been a matter of educated guesswork to select which factors to study. Nevertheless, many can be missed, so we have developed a method to screen for unanticipated cytokine expression. EXPERIMENTAL DESIGN: Amplification products from polymerase chain reactions (PCR) of different cytokine genes were spotted onto nylon membranes, and probed with radioactive cDNAs made from mouse endothelioma cells. RESULTS: The presence of interleukin-3 was detected, and confirmed by conventional Northern blotting and polymerase chain reaction (PCR). CONCLUSIONS: This method may be useful to survey cells for the expression of cytokine genes.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1995
Accession Number
ADA304203

Entities

People

  • Florence M. Rollwagen
  • Henry Chang
  • Paul D. Siebert
  • Thor B. Nielsen
  • Ying-yue Li

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Amplification
  • Chain Reactions
  • Chemical Reactions
  • Cytokines
  • Experimental Design
  • Membranes
  • Polymerase Chain Reaction
  • Surveys

Fields of Study

  • Chemistry
  • Medicine

Readers

  • Immunology and Pathology
  • Molecular Genetics
  • Systems Analysis and Design