Development of Novel Peptide Inhibitors of the Estrogen Receptor

Abstract

This project aims to test the hypothesis that polypeptides designed to mimic the dimerization domain of the estrogen receptor are capable of serving as inhibitors of receptor function by virtue of their ability to interfere with the binding of receptor protein to DNA. During the first year of this award, peptides have been acquired and assays have been developed to initiate this project. Several candidate peptides have been identified that appear to specifically block the DNA-binding activity of the estrogen receptor as assessed using an in vitro gel mobility shift assay. These observations will be confirmed and extended during the final year of this project by assessing whether or not this inhibitory activity can be reproduced in a cell culture model system of estrogen receptor action. Due to a change in the institutional affiliation of the PI (Dr. R.J. Miksicek), requests have been filed to permit a change in the performance site for this project as part of a subcontracting arrangement. This request is currently pending with the US Army MRDC.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1995
Accession Number
ADA304750

Entities

People

  • Richard J. Miksicek

Organizations

  • State University of New York

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Computer Networks
  • Culture Techniques
  • Cultured Cells
  • Estrogens
  • Genetic Code
  • Hormones
  • Inhibitors
  • Medical Personnel
  • Mobility
  • Neoplasms
  • New York
  • United States

Readers

  • Molecular Genetics
  • Prostate Cancer Biology.
  • Technical Research and Report Writing.