Genetic Evidence of Early Breast Cancer.

Abstract

The earliest phases of breast cancer are poorly characterized. A precise understanding of the genetic changes which herald incipient transformation would be invaluable for accurate early diagnosis and a better understanding of its pathogenesis. Towards this goal, we aim to identify clonal breast populations based on the detection of only one X-chromosome androgen receptor allele after differential methylation sensitive restriction enzyme digestion. Although this analysis is suitable for large amounts of DNA, it was not reliable when small cell numbers were analyzed. Since the earliest breast cancer lesions are small, we have yet to develop a suitable assay for their analysis. However, new efforts based on technical improvements show promise for both high sensitivity and reliability.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1995
Accession Number
ADA305889

Entities

People

  • Darryl Shibata

Organizations

  • University of Southern California

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Androgens
  • Biomedical Research
  • Breast Cancer
  • Cells
  • Chemical Reactions
  • Chromosomes
  • Digestive System Processes
  • Genetic Phenomena
  • Genetic Structures
  • Genetics
  • Materials
  • Methylation
  • Molecules
  • Neoplasms
  • Polymerase Chain Reaction
  • Reliability

Fields of Study

  • Biology

Readers

  • Molecular and genetic basis of cancer.
  • Systems Analysis and Design

Technology Areas

  • Biotechnology