Molecular Biological Approaches to Studying Cyanide Detoxification by Rhodanese.
Abstract
Rhodanese (thiosulfate sulfurtransferase) is expressed as high levels in liver and involved in the detoxification of cyanide. The full-length cDNA corresponding to the mouse rhodanese gene (Tst), which is located on chromosome 15, was cloned by PCR amplification of a liver cDNA library and subjected to DNA sequencing. Alignment of the rhodanese cDNA sequences from mouse and rat, which we previously cloned (Biochem. J. 275:227-231 revealed 97.3 percent identity at the protein level and 94.6 percent identity at the DNA leve When the mouse and rat cDNAs were expressed under the control of IPTG-inducible promoters in E. Coli, the cell extracts exhibited cyanide-metabolizing activity, indicating that both genes encode functional rhodanese molecules. The results of the study were published by Dooley al. in Biochemical and Biophysical Research Communications 216:1101-1109 (1995).
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1995
- Accession Number
- ADA306911
Entities
People
- Thomas P. Dooley
Organizations
- Texas Biomedical Research Institute