X-Ray Crystallographic Studies on Acetylcholinesterase and on its Interaction with Anticholinesterase Agents.

Abstract

The objective of this project was to solve, for the first time, by X-ray crystallography, the three-dimensional structure of acetylcholinesterase (AChE) and to learn from this structure the reaction mechanism, inhibition modes and other structural and functional properties of the enzyme. The principal objectives of the current project included: (1) Attempts to obtain crystals of cholinesterases other than T. californica AChE, in particular of AChE of human origin. (2) Collection of improved data sets, viz. at higher resolution than previously, for crystals of Torpedo AChE. (3) Determination of complexes of Torpedo AChE with various ligands, including orgamophosphates. In the this project, we have made progress towards all three objectives, as well as in additional areas related to the long-term aims of our research program

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1995
Accession Number
ADA307114

Entities

People

  • Israel Silman
  • Joel L. Sussman

Organizations

  • Weizmann Institute of Science

Tags

Communities of Interest

  • Weapons Technologies

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Alzheimer Disease
  • Amino Acids
  • Cells
  • Chemistry
  • Crystal Lattices
  • Crystal Structure
  • Crystallography
  • Crystals
  • Data Sets
  • Diseases And Disorders
  • Enzyme Inhibitors
  • Laboratory Animals
  • Molecular Dynamics
  • Poisoning
  • Three Dimensional
  • X Rays

Fields of Study

  • Chemistry

Readers

  • Computational Modeling and Simulation
  • Materials Science and Engineering.
  • Neurotoxicology