Construction of a Recombinant Viral Vector Containing Part of the Nucleocapsid Protein Gene of Newcastle Disease Virus,

Abstract

This report describes the procedures used to clone a 673 base pair gene fragment of the major nucleocapsid protein gene of Newcastle disease virus into a viral vector molecule for the purpose of maintaining a stable, long-term, renewable source of this target sequence for gene probe studies. The gene fragment was prepared by reverse-transcription polymerase chain reaction of Newcastle disease virus RNA and was cloned into the viral DNA vector Ml3mp18 RF to produce a recombinant DNA molecule. The cloned fragment was shown to be present in the recombinant clones based on (i) clonal selection on indicator plates; (ii) restriction enzyme analysis; (iii) gene probe analysis and (iv) nested PCR amplification.

Document Details

• Document Type: Technical Report
• Publication Date: Sep 01, 1995
• Accession Number: ADA307322

Entities

People

• Douglas E. Bader

Organizations

• Defence Research and Development Canada

Tags

DTIC Thesaurus Topics

• Acids
• Alcohols
• Biological Factors
• Chemical Reactions
• Chemistry
• Deoxyribonucleic Acids
• Gene Expression
• Indicator Dyes
• National Security
• Nucleic Acids
• Polymerase Chain Reaction
• Recognition
• Recombinant Dna
• Ribonucleic Acids
• Schematic Diagrams
• Thermal Cyclers
• Viruses

Fields of Study

• Biology

Readers

• Molecular Genetics
• Oncology