Analysis of the Regulation of Expression of Transforming Growth Factor-Beta in Human Breast Cancer Cells.

Abstract

This grant entails two objectives. These objectives derived from prior work by ourselves and others which have identified TGF-B as an important cytokine in the biology of breast cancer. For established tumors, overexpression of TGF-B may result in increased in vivo tumor growth and metastatic spread. The first objective involves the analysis of resected breast cancer specimens, with the ultimate goal of determining the mechanism(s) by which TGF-B could be overexpressed by some tumors. There are as yet no definitive data or conclusions from this aspect of the work. The second objective of the grant was to identify the molecular determinants of promoter usage for TGF-B3 in breast cancer cells. These studies stem from our prior work in which we have characterized an alternative TGF-B3 promoter uniquely functional in breast cancer cells. We have made some progress in designing promoter constructs which yield authentic promoter function upon transfection into breast cancer cells. The Statement of Work did not call for any conclusions by this point in the grant, and none are available.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1995
Accession Number
ADA308053

Entities

People

  • Bradley A. Arrick

Organizations

  • Dartmouth College

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Biomedical Research
  • Breast Cancer
  • Deoxyribonucleic Acids
  • Diseases And Disorders
  • Growth Factors
  • Materials
  • Molecules
  • Neoplasms
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • Recombinant Dna
  • Regulations
  • Standards
  • Statistical Analysis
  • Transfection

Fields of Study

  • Medicine

Readers

  • Oncology (Cancer Research).
  • Systems Analysis and Design