Two-Dimensional Protein Pattern Recognition in Chemical Toxicity.
Abstract
The mechanisms underlying the effects of chemical toxicity such as cell damage, necrosis, and death, as well as repair, involve changes in the abundance and qualitative character of specific proteins in target tissues and relevant in vitro systems. One way to detect such toxic effects, to determine the mechanism(s) involved in a specific effect among a broad range of cellular mechanisms, and to predict the effects of structurally similar compounds, would involve a system that enables simultaneous analysis of greater than 1000 target cell proteins with respect to their response to chemical intoxication. Recognizable patterns of protein alteration so documented would serve as biomarkers of injury or repair, or both. This final technical report documents the progress made during the research period regarding the application of large-scale two-dimensional electrophoresis (2DE) and image analysis to Air Force Toxicologic interests and describes, in particular, the development of prototype tissue 2DE protein databases; multi-species, multi-tissue, and serum 2DE protein map development; and stress protein biomarker development in rodent and human liver samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 15, 1996
- Accession Number
- ADA309744
Entities
People
- Frank A. Witzmann