In Vivo Studies of Hematopoietic Growth Factors for Use in Military Personnel.
Abstract
The secondary structures and thermal denaturation/renaturation of recombinant human erythropoietins were compared using UV circular dichroism. Marked differences were observed between proteins expressed by different host cell lines, presumably due to dissimilar glycosylation patterns. A pharmacokinetic study of monomeric erythropoietin carried out in baboons demonstrated a very short plasma half-life of three hours. The proper formulation of the protein for these studies was established. Work on erythropoietin +dimers progressed, but high yields in each reaction were different to achieve. Knowledge of the differing glycosylation patterns will help improve these yields. Work on erythroid colony stimulation factor was resumed, and rodent bone marrow cultures were established.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 25, 1995
- Accession Number
- ADA311176
Entities
People
- Arthur J. Sytkowski
Organizations
- Beth Israel Deaconess Medical Center