Autoradiographic Localization of (125I)-Ricin in Lungs and Trachea of Mice Following an Aerosol Inhalation Exposure.
Abstract
Studies were conducted to examine the light microscopic distribution of (1251)-ricin within the lungs and corresponding lung pathology in mice following single, nose-only, aerosol inhalation exposures. Groups of 4-8 mice were euthanatized 15 minutes and 1, 4, 6, 8, 12, and 24 hours post-exposure. At 15 minutes post-exposure, abundant radiolabel was present within alveolar spaces. At all later times, however, (125l)-ricin was observed primarily within the lumina of bronchioles and larger passageways. Cellular targets for ricin within the lungs could not be identified using the techniques employed; it is therefore recommended that ultrastructural autoradiography be used to determine cellular ricin binding targets. The primary pathological abnormality detected was multifocal epithelial necrosis of the secondary bronchioles.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1996
- Accession Number
- ADA311764
Entities
People
- James E. Estep
- Jeffrey A. Doebler
- Norman D. Wiltshire
- Robert B. Moeller
- Thomas W. Mayer
Organizations
- United States Army Medical Research Institute of Chemical Defense