Identification of Nucleic Acid Sequences and Chemical Markers for Taxonomic Characterization of Bacteria.
Abstract
Our biodetection research is primarily concerned with two inter-related issues (1) development of novel schemes for improving chemotaxonomic characterization of microbial pathogens and (2) mass spectrometry-based methods for trace detection of chemical markers for key agents in complex environmental matrices. $180,000 in direct + indirect costs were provided over the period June 1992-1995. This contract was extended with additional funds of $49,020 from ERDEC through June 1995- June 1996. Development of chemotaxonomic schemes for correct identification of species of bacteria relevant to the biodetection program is essential. The research employed a systematic search for new chemical markers employing state-of-the-art chemical and molecular approaches. The feasibility of detecting markers for bacteria in environmental samples was demonstrated by developing a working method for detection of muramic acid (universally found in bacteria) in airborne dust. A prototype approach for the rapid (< 10 minutes) detection/identification of microorganisms (B. anthracis) based upon the combination of polymerase chain reaction (PCR) and electrospray mass spectrometry is also described. Extrapolation of this approach to environmental monitoring would represent a major improvement over existing technologies for biodetection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 14, 1996
- Accession Number
- ADA313737
Entities
People
- Alvin Fox
Organizations
- University of South Carolina