Heregulin-Induced Growth Factor Receptor Signaling and Breast Carcinogenesis.

Abstract

We have engineered a panel of Ba/F3 cell lines that express, singly and in every pairwise combination, the four erbB family receptors. Using this panel of cell lines, we have evaluated hormone-induced erbB family receptor phosphorylation and coupling to downstream signaling proteins and physiologic responses. To date, we have tested six epidermal growth factor (EGF) family hormones: EGF, transforming growth factor alpha (TGFcc), heparin-binding EGF-like growth factor (HB-EGF), amphiregulin (AR), betacellulin (BTC), and neuregulin-beta (NRGB), also known as heregulin or neu differentiation factor. EGF, BTC, and NRGB exhibit distinct activities, while EGF, TCFQ and HB-ECF are functionally equivalent. Furthermore, the four erbB family receptors exhibit differential coupling to cellular signaling proteins and physiologic responses. This suggests that cellular responses to activation of the EGF family/erbB family signaling network are specified by several hierarchical mechanisms.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1996
Accession Number
ADA315700

Entities

People

  • David F. Stern
  • David J. Riese

Organizations

  • Yale University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Breast Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Cultured Cells
  • Epithelial Cells
  • Growth Factors
  • Health Services
  • Mammary Glands
  • Medical Personnel
  • Neoplasms
  • Peptide Growth Factors
  • Peptides

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.