New Multilabel Fluorescent Groups for Increased Sensitivity of DNA Detection.

Abstract

The long-term goals of this project are to use oligonucleotides tagged with multiple fluorescent labels as hybridization probes of specific nucleic acid sequences. We have developed novel modes of binding between designed DNA probes and target DNA or RNA sequences, and we are investigating the combination of these new binding modes with dual (or more) fluorescent labels to raise signal intensity and signal-to-noise ratios. Such probes may be useful in detection and identification of pathogen nucleic acids as well as disease-related nucleic acid sequences in humans.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1996
Accession Number
ADA316448

Entities

People

  • Eric T. Kool

Organizations

  • University of Rochester

Tags

DTIC Thesaurus Topics

  • Acids
  • Biomolecules
  • Chemical Compounds
  • Chemistry
  • Detection
  • Discrimination
  • Engineering
  • Fluorescence
  • Identification
  • Intensity
  • Laser Dyes
  • Molecules
  • Nucleic Acid Probes
  • Nucleic Acids
  • Nucleosides
  • Recognition
  • Sequences

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular Genetics
  • Radar Systems Engineering.

Technology Areas

  • Biotechnology