Identification of Brucella Melitensis Antigens by T Lymphocytes and Oral Immunization Potential of the Antigens.
Abstract
Acquired immunity against intracellular bacteria is T cell dependent. T cells play a major role in protection against intracellular bacteria, but bacterial antigens recognized by T cells have been studied less extensively than bacterial antigens recognized by B cells. Using T lymphocytes from Brucella abortus immunized animals, we have screened a bacterial genomic library for genes encoding antigens recognized by T cells. Lymphocytes that proliferated to B. abortus proteins were characterized for phenotype and cytokine activity. Lymphocytes recognizing bacterial antigens possessed type 1 cytokine profiles, i.e., IFN-gamma and IL-2 but not IL-4 production. Lymphocytes necesssary for In vivo protection were examined using gene knockout mice. MHC class I and class II gene knockout animals infected with B. abortus demonstrated that protection to B. abortus is especially dependent on CD8+ T cells. Knowing the cells required for protection, vaccines can be designed to elicit the protective subset of lymphocytes. Several recombinant B. abortus proteins were tested using different immunization strategies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1996
- Accession Number
- ADA317023
Entities
People
- Gary Splitter
Organizations
- University of Wisconsin–Madison