Stromal Components of Breast Cancer Progression.
Abstract
Metastatic estrogen receptor positive breast carcinoma may be treatable with tamoxifen, an antiestrogen, but the tumor may subsequently become refractory to treatment, growing in the absence of estrogenic stimulation. We have developed a model of breast cancer progression by transfecting MCF-7 breast carcmoma cells, which are estrogen-dependent for growth in ovariectomized nude mice, with cDNAs for FGF-I or FGF-4. The transfected cell lines are able to form tumors in ovariectomized nude mice. Since FGFs are angiogenic factors, this project investigates the importance of angiogenesis in the phenotypic transition of the transfected cells by looking for differences in the angiogenesis in tumors produced by the parental MCF-7 or the FGF-transfected cells. Our model is validated by a positive correlation of tumor microvessel density and tumor size. We have defined temporal and spatial events in the process of tumor-induced angiogenesis by identifing sprouting or proliferating endothelial cells. We have identified patterns of angiogenesis which are associated with regressing parental cell tumors and growing FGF-transfected cell tumors. We have begun to isolate endothelial cell populations from parental or FGF-transfected cell tumors to study FGF receptor gene expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1996
- Accession Number
- ADA317374
Entities
People
- Sandra W. Mcleskey
Organizations
- Georgetown University