Augmentation Award for Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments.
Abstract
The nonextractive immunoassay techniques developed with enzyme immunoassay procedures were extended to include fluorescent immunoassay of sorbed contaminants. The variety of substrates to which the contaminant was sorbed was increased to include: St. Peters sandstone, montmorillonite, and black shale. Auto-fluorescence of the substrates was addressed, and 2,4-dinitro-benzene was found not to migrate into the montmorillonite crystal layers. Additional sample preparation was needed for the clay samples: significantly more blocking solution was needed to reduce background nonspecific binding of the immunochemicals. Two contaminants were used throughout the grant period -- 2-4-dinitrobenzene, and pyrene.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 31, 1996
- Accession Number
- ADA318009
Entities
People
- Deborah J. Mossman O'bannon