Role of the Int-3 Oncogene in Mammary Gland Development and Tumorigenesis.
Abstract
The overall goal of the work proposed here is to understand in molecular detail the function of the int-3 protein in mammary epithelial cells and during mouse mammary gland development. The approach I propose will have two major objectives. First, the biochemical and biological properties of the int-3 protein will be investigated. I have cloned the part of the int-3 gene that encodes for the putative intracellular domain of the int-3 protein, which is thought to be constitutively active. Initially, this truncated protein will be used as a probe to study the signaling pathway and the mechanisms of action of the int-3 protein. These studies will be further expanded once the full-length int-3 is cloned. Second, I will clone, sequence and characterize the full length int-3 cDNA and study int-3 function and expression pattern in mammary epithelial cells as well as during mammary gland development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADA319341
Entities
People
- Hendrik Uyttendaele
- Jan Kitajewski
Organizations
- Columbia University