Genomic Instability at Premalignant and Early Stages of Breast Cancer Development.
Abstract
We have developed techniques that allow the analysis of multiple chromosomal loci from single paraffin sections from breast cancer lesions. This approach is being used to allelotype small preinvasive breast cancer lesions. We identified the chromosome arms most frequently affected by allelic losses and imbalances at preinvasive stages of breast carcinogenesis and those allelic losses involved in more advanced stages of progression. We have now performed a high resolution allelotype of chromosome 16q. This allowed us to refine the location of specific subchromosome regions containing putative tumor suppressor genes of relevance on early breast carcinogenesis. We also observed that microsatellite instability (replication error phenotype, RER+) is characteristic of a subset of breast tumors (invasive lobular breast carcinomas), indicating the possible involvement of defective DNA mismatch repair genes in these tumors. Studies are ongoing to address this point and to evaluate the use of RER phenotype determination as a prognostic-diagnostic tool.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1996
- Accession Number
- ADA319734
Entities
People
- C M Aldaz
Organizations
- The University of Texas MD Anderson Cancer Center