Mammary Tumor Development: Stromal-Epithelial Interactions in Oncogenesis.
Abstract
The purpose of this grant is to examine interactions between stroma and epithelium in mammary oncogenesis in trarisgenic mice that expressing Shope growth factor (SGF). Different lines of SGF transgenic mice express this EGF-like cytokine using inducible (metallothionein, MT) and constitutive (RSV-LTR) promoters. We have fulfilled and surpassed stated goals for year 01. The first project year proposed producing sufficient SGF-transgenic mice from RSV-SGF and MT-SGF lines for analysis of mammary development and tumorigenesis, including measuring SGF expression and expression of genes associated with mammary cancer in humans and animals. Availability of sufficient mice has permitted us to begin these histologic and molecular analyses. We have, further, made functional anti-SGF antiserum. Breeding between RSV- and MT- SGF mice, and p53- mice, has begun, to address experimentally the strong association of p53 mutations with human breast cancers, and to test whether this association holds for experimental mammary carcinogenesis in this system. Thus, we have initiated new studies that are progressing well, and progress in pursuing specific aims is on or ahead of schedule. We expect this project to be completed as scheduled.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADA319825
Entities
People
- David S. Strayer
Organizations
- Thomas Jefferson University