Suppression of Lymphocyte Signal Transduction by Oxygen Intermediates.
Abstract
Oxidative stress alters the expression of transcription factors in human lymphocytes. Given the critical role these factors play in gene regulation, their abnormal regulation should lead to disturbances in gene expression and these in turn will result in cellular dysfunction. The transcription factor abnormalities may be used to develop an assay for the detection of environmental toxic oxidants. Technical objectives: To study three models of inducing oxidative stress in lymphocytes: (a) PAO activity generating extracellularly low levels of H2O2 for two days (mimicking exposures to environmental chemical toxicants); (b) electron irradiation generating both extra and intracellularly mainly OH; and (c) high levels of reagent H2O2 generating short but acute stress. The following questions were asked during the first year of work: Do human lymphocytes subjected to oxidative stress: (a) exhibit abnormal cellular function-expression of transcription factors that regulate the interleukin 2 gene (essential for T lymphocyte function)?; and (b) sustain damage as assessed by an independent and established method: measurement of lipid peroxidation?
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1996
- Accession Number
- ADA319847
Entities
People
- Eliezer Flescher
Organizations
- NYU Langone Health