Establishment of a Repository for Cell Cultures and Genomic DNA from Breast Cancer Patients.
Abstract
New breast cell lines and cell strains were developed from breast cancer tissues, uninvolved tissues from cancer patients and normal tissues from reductive mammoplasty patients. Two different approaches were tried to generate breast cell lines with malignant properties: (a) transfect the breast cells with a plasmid containing mutated ras oncogene and (b) treat a normal (non-malignant) breast cell with a mutagenic chemical in vitro. All the cell strains/lines developed were added to the breast cell repository. Seventeen cultures passed through the second subculture and were cryopreserved. Of seventeen, six were classified as cell lines and the remaining eleven as cell strains. Some of the established cell lines have been partially characterized with respect to karyotype, the presence of the H-ras and p53 genes, expression of specific gene markers, and cumulative population doubling levels. Preliminary results show significant cytotoxic effects on an established cancer cell line by two anticancer agents, taxol and 5-fluorouracil. Additional cell cultures will be developed and characterized during the grant extension period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1996
- Accession Number
- ADA319903
Entities
People
- P. T. Type